Curriculum Vitae

Annette M. Golonka

USC Lancaster, 476 Hubbard Drive, Lancaster, SC 29720

June 5, 2013

Duke University, Department of Biology, Durham, North Carolina
  PhD Thesis Advisor Janis Antonovics (currently at University of Virginia)
Co-Advisor Rytas Vilgalys at Duke University
 "Nectar-Inhabiting Microorganisms (NIMs) and the dioecious plant species Silene latifolia"
PhD in Botany, Area: Population Ecology, December 2002
Certificate in Ecology from the University Program in Ecology December 2002
Certificate in Teaching College Biology December 2002
University of California, Irvine, California
  BS in Biology, Cum Laude, June 1996
Specialization in Ecology
Brief Teaching Philosophy
     I teach by interactive lecturing, asking students questions as I lecture, getting them involved in the lecture, and making them part of the learning process. Initially, students may be hesitant about answering my questions, but during the semester they learn that I enjoy the interaction, and I do not tolerate ridicule of an incorrect answer. In this way, the classroom becomes a place where they enjoy being and learning about biology. My teaching style is driven not only by a need to teach students about biological concepts and how to apply these concepts, but also by a desire to understand my students, to learn from them, and to become a better educator in the process. I want students to view me as both a mentor and a source of biological knowledge.

Academic Employment
  Assistant Professor of Biology , USCLancaster, Aug 2006-present

Teach the following courses:
BIOL 101 - Biological Principles I (Biology majors course)
BIOL 302/302L - Cell & Molecular Biology lecture and lab (Biology majors course)
BIOL 303 - Fundamental Genetics (Biology majors course)
BIOL 250/250L - Microbiology (nonmajors course, nursing course, pre-health, pre-pharmacy)

  Microbiology Lab Administrator / Instructor, Duke University, Aug 2003 - July 2006

General Microbiology (Bio 103L) for undergraduates.
Coordinate and perform activities in support of the introductory microbiology lab course, a majors and non-majors course. Duties include: training and supervising teaching assistants; editing course lab manual; creating and evaluating prototype labs to enhance the curriculum; deciding which labs to implement during the semester; and supervising the set-up of lab materials; occasional lecturing in the course; and maintenance of the course website and Blackboard content accessible by guests (, click on "log in", then "Courses" tab, then search for Bio 103).

  Teaching Assistant, Duke University, Sept 2000 - Dec 2002

Introductory Microbiology for undergraduates.
Conducted biweekly laboratory sections: lectured at beginning of labs, wrote and graded lab quizzes and lab practical, and graded lab papers. For lecture: proctored and graded exams, gave substitute lectures, wrote exam questions, and maintained website for lecture and lab.

  Aide, Duke University, June - July 2002

Aide for the Pre-College Howard Hughes Program working with high school students.
Helped with a week-long laboratory program, and acted as liaison between students and participating laboratories around Duke's campus.

  Teaching Assistant, Duke University, Sept 1996 - May 1997

Introductory Biology.
Led discussion and laboratory meetings, graded lab papers, proctored and graded exams.

  Assistant I, University of California, Irvine (UCI), June 1994 - Aug 1996

Science Education Programs for K-12 school teachers, Department of Chemistry.
I worked as a teacher's aide for a program offered during UCI's Summer Science Institute (the California Science Project/Orange County Science Education Network) for elementary and secondary education teachers.

Professional Development
  McGraw-Hill Higher Education On-Line Workshop ,USCLancaster, 2009
    Workshop entitled "Integrating active learning into your microbiology course" to help faculty use technology in their courses.
  Association for Biological Laboratory Education Conferences , attended in 2007 & 2008
    Workshops on teaching different lab exercises. Hands-on laboratory experiences.
  Instructional Technology Showcase Conference, Duke University, Spring 2006
    Presentations, speakers, and poster sessions focused on the accomplishments of duke faculty using technology in teaching. Topics included information on new features available on-line through Blackboard as well as other teaching programs and websites. Introduction to computer based simulations as teaching tools for subjects ranging from economy to medical training
  Teaching is the Art of Changing the Brain, Duke University, Spring 2006
    Seminar by James Zull, author of The Art of Changing the Brain, Professor of Biology and Biochemistry and Director of the University Center for Innovation in Teaching and Education at Case Western Reserve University. Presentation on how the brain learns and why visual aids are necessary.
  Teaching for Understanding: Active Learning and Assessment, Duke University, Fall 2004
    Workshop by Diane Ebert May, Professor of Plant Biology, Michigan State University.
Questions addressed included: How can you run a large class and never lecture? And why is this a good idea? What do you do when your students have major misconceptions about your subject? And how can you be sure that your students actually learn anything?
  Certificate in Teaching College Biology, Duke University, Spring 2002
    Seminar in teaching Biology, Bio 390, Instructor Paula Lemons, PhD.
A seminar course on all aspects of how to teach biology including media usage, grading and testing, classroom dynamics, course development, and inquiry based teaching.
  Faculty Mentorship, Duke University, Fall 2001 - Dec 2002
    My mentor: Sandra Seidel, an Associate Professor of Biology at Elon University, currently at University of Virginia.
Meetings included discussions on how to teach non-majors, how to design a course, where to find information for courses, dynamics in the classroom, balancing content and quality of content. This is still an on-going mentorship.

Scholarships, Grants, and Honors
    PI on a Magellan Scholar Award with Dr. Bettie Obi Johnson as Co-PI, Spring 2013, with sophomore Austin Blackwell.
    Co-PI on a Magellan Scholar Award with Dr. Bettie Obi Johnson as PI, Spring 2011, with Sophomore Jonathan Freeman
    Mini Faculty/Staff Research and Productive Scholarship, USCLancaster, 2010-2011.
    Association for Biological Laboratory Education (ABLE) tuition waiver, June 2007, for ABLE Conference at University of Kentucky.
    Mini Research and Productive Scholarship, USCLancaster, 2007.
    Co-PI on "Continuous Renewal of Undergraduate Education Grant via an Interdisciiplinary, Inquiry Based Laboratory", NSF #0633648.
  Duke University
    Center for Instructional Technology Jump Start Grant with Dr. Xinnian Dong, Spring 2005. Funds to develop an instructional website focusing on genomics and its usage in microbiology.
    Catherine Keever Award. Supplies for research at MLBS, June 2000-2001.

Mountain Lake Biological Station, University of Virginia Gift Grant. Support for room and board. June 2000.

    Sigma Xi Grant-in-Aid, June 1997. Funds to conduct research at MLBS.

NSF Graduate Research Fellowship, June 1997 - 2000.

  University of California, Irvine
    Honorable Mention NSF Graduate Research Fellowship Awards, June 1996.
    NSF Research Experience for Undergraduates (REU), June 1995 - Aug 1995
    Golden Key National Honor Society, 1994 - present.
    National Science Scholars Program, 1992-1995.
    Deans Honor List (11 quarters), 1993-1995.
    Orange County Teachers Federal Credit Union Scholarship, 1992-1993.
    Garden Grove Association of American University Women Scholarship, 1992-1993.
    Italian Catholic Federation Scholarship, 1992-1993.

Research Interests
My research interests focus around microbial ecology, diversity, and evolution as well as plant ecology, spanning disciplines. I am interested in population and community dynamics of plants, the interaction of microbes with plants and pollinators, yeast evolution and ecology, the evolution of gender in plants, and pollination biology.

Research Experience
  Current Research, USCLancaster, 2006-present

I work on local plant species, such as Silene caroliniana and Gelsemium sempervirens. The work I do tends to be microbially oriented. I study the diversity of nectar-inhabiting microorganisms in these plant species as well as the volatile organic compounds produced by these plant species in collaborative research with our analytical chemist, Dr. Bettie Obi Johnson.

  Dissertation Research, Duke University, 1996 - Dec 2002

I examined the presence and diversity of nectar-inhabiting yeasts in Silene latifolia, a dioecious plant species (separate male and female individuals), and investigated the interactions between plants, pollinators, and yeast.   I isolated 28 species of yeast from S. latifolia and associated plant species, and identified them using DNA-based phylogenetic analyses and morphological techniques.  There was evidence of both host specificity and widespread dispersal of yeast species.  There were no consistent differences among the sexes of S. latifolia, but male plants appeared to have a lower diversity and species richness in some years than female plants.  Eight yeast species were examined in regard to their growth in nectar and sugar solution.  Several species were capable of growing in high sugar concentrations or water, but far fewer grew in real nectar and more species grew in female nectar than male nectar.  Hand-pollination experiments on female flowers inoculated with yeast species (Candida bombi and Metschnikowia reukaufii) indicated no direct effect of these microorganisms on seed production; however, there is some indication of indirect effects (i.e., pollinator visitation patterns).

  Undergraduate Research Project, University of California, Irvine. Jan 1995 - Aug 1996

Undergraduate research on the morphology of Schiedea and Alsinidendron (supervised by Drs. Ann Sakai and Stephen Weller, funded in part by a NSF Research Experience for Undergraduates).
I investigated sexual dimorphism in the Hawaiian genus, Schiedea, with emphasis on morphology (including primary and secondary sex traits), nectar volume, and concentration of nectar sugars.  I compared dimorphic species of Schiedea with hermaphroditic species to determine how floral morphology has changed with the evolution of wind pollination and dimorphic breeding systems (gynodioecy, subdioecy, and dioecy).   Results of some of this work have been published.

Skills and Techniques
Ecology: Pollination biology techniques, nectar analysis (including extraction, volume quantification, and sugar content determination), species diversity indices, analysis of categorical data, species identification of plants and some insects, and other ecological methods used in the field.
Microbiology: Culture and staining techniques for bacteria and yeast, selective media usage, bacteria and yeast identification procedures, and basic microscopy, including fluorescence microscopy.
Molecular Biology: DNA extraction, polymerase chain reaction, DNA sequencing, transformation, cloning, PCR identification of bacteria and yeast, and phylogenetic analysis and reconstruction.

Computer skills: PC, MAC, and UNIX familiar; statistical software - SAS, JMP, some SPLUS; phylogenetic programs - PAUP, Genedoc, Clustal, DAMBE; spreadsheet software - Excel and Systat; graphics software - SigmaPlot, Adobe Illustrator, Ulead PhotoImpact; HTML designing software - HotDog, DreamWeaver; miscellaneous software - Word, Powerpoint, Netscape, IExplorer, Blackboard.

Scholarly Publications and Presentations

Peer-Reviewed Journal Articles:
Golonka, A. M. and R. Vilgalys. 2013. Nectar inhabiting yeasts in Virginian populations of Silene latifolia (Caryophyllaceae) and coflowering species. The American Midland Naturalist. 169: 235-258. (pdf available upon request)

Golonka, A. M., A. K. Sakai, and S. G. Weller.   2005. Wind pollination, sexual dimoprhism, and changes in floral traits of Schiedea (Caryophyllaceae).   American Journal of Botany.  92: 1492-1502.

Weller, S. G., A. K. Sakai, A. E. Rankin, A. Golonka, B. Kutcher, and K. E. Ashby.  1998.  Dioecy and the evolution of pollination systems in Schiedea and Alsinidendron (Caryophyllaceae: Alsinoideae) in the Hawaiian Islands.  American Journal of Botany.  85:1377-1388.

Golonka, A. M.  1996.  Sexual Dimorphism in Schiedea (Caryophyllaceae). Journal of Undergraduate Research in the Biological Sciences.  University of California, Irvine.  26: 160-166.

Oral and Poster Presentations (*=undergraduate while at USCL):
*Blackwell, Austin, Annette Golonka, and Bettie Obi Johnson. The sweet smell of Carolina jessamine: evaluating the floral scent profile of Gelsemium sempervirens. University of South Carolina Discovery Day, poster, April 26, 2013. First place in poster session.

Golonka, Annette, Bettie Obi Johnson, Jonathan Freeman, and *Daniel Hinson. Production of volatile organic compounds by two Metschnikowia eyasts isolated from Silene caroliniana nectar. South Carolina Branch Meeting of the American Society of Microbiology,poster, April 20, 2013.

*Freeman, Jonathan, Bettie Obi-Johnson, and Annette Golonka. Evaluation of the impact of yeast on floral scent in Silene caroliniana. South Carolina Academy of Sciences National Meeting, poster. April 14, 2012.

Golonka, Annette, Bettie Obi Johnson, *Jonathan Freeman, and *Daniel Hinson. Determination of volatile compounds produced by yeasts inhabiting the nectar of Silene caroliniana (Caryophyllaceae). Association of Southeastern Biologists Meeting, orakl presentation. April 6, 2012.

*Freeman, Jonathan, Annette Golonka, Bettie Obi-Johnson. The role of nectar inhabiting yeast in the floral scent of Silene caroliniana plants. University of South Carolina Discovery Day, poster. April 22, 2011.

Teaching Materials:
Golonka, A. M. Fall 2007 through Spring 2013 Semester Issue. Microbiology, Biol L250L. Unviersity of South Carolina Lancaster, Lancaster, SC.

Golonka, A. M. and R. Vilgalys.  Fall 2004 through Spring 2006 Semester Issue.   Laboratory Exercises for Bio 103 General Microbiology.  Duke University, Department of Biology, Durham, NC.

Guest Speaker :
For Sun City Lifelong Learning Program -- "DNA Fingerprinting", hour long presentation, March 2008.

For Sun City Lifelong Learning Program -- "Human Genome Project", hour long presentation, Nov 2007.

Manuscripts in Preparation (*=undergraduates while at USCL):
Golonka, A. M., B. Obi Johnson, *J. Freeman, and *D. Hinson. Nectarivorous yeasts and Silene caroliniana's scent profile.

Golonka, A. M., and *D. Hinson. Nectar inhabiting microorganisms in flowers of Silene caroliniana (Caryophyllaceae).

Golonka, A. M.   Differential growth rate of nectar-inhabiting microorganisms in male versus female nectar of Silene latifolia.

Golonka, A. M., and J. Antonovics.   The effect of nectar-inhabiting yeast on maternal fitness of Silene latifolia.

Talks prior to USCL

Dec 2002 Thesis Seminar at Duke University - "Diversity of Nectar-Inhabiting Microorganisms and Interactions with Silene latifolia"
March 2002 Ecology, Evolution and Organismal Biology Graduate Symposium at Duke University - "Diversity of Nectar-Inhabiting Microorganisms in Silene latifolia"
Nov 2001 Population Biology Group at Duke University "Nectar-Inhabiting Microorganism Diversity and Plant Fitness or how I spent the last 4.5 seasons"
June 2001 Mountain Lake Biological Station, Va. "The Life of a Nectar-Inhabiting Microorganism"
June 2000 Society for the Study of Evolution meetings "Studying the Potential Effects of Nectar Inhabiting Microorganisms (NIMs) on Plant Fitness"
April 2000 Mid-Atlantic States Mycology Conference (MASMC) held at Duke University "Ecological Dynamics of Yeast in Nectar"
Oct 1999 Population Biology Group at University of Virginia "Nectar Creatures and What has Gender got to do With it!"
Sept 1999 South Eastern Ecological, Population Genetics and Evolution (SEEPAGE) Conference held at Mountain Lake Biological Station, Va "Nectar-Inhabiting Microorganisms (NIMs) and Silene latifolia (= S. alba)"
Feb 1999 Population Biology Group at Duke University "What is the relationship between nectar-inhabiting microorganisms (NIMs) and the dioecious plant, Silene alba?"
Oct 1998 Evolutionary Resynthesis Mini-Symposium at Duke University "Introduction to Nectar-Inhabiting Microorganisms (NIMs)"

Posters and Presentations Prior To USCL
June 1999 Guest speaker for Howard Hughes Program for High School Students at Duke University Interactive presentation on how to measure nectar volume and sugar concentration of different flowers, and what types of microorganisms might inhabit floral nectar.
June 1999 Society for the Study of Evolution Meetings; Poster
"Incidence of Nectar-inhabiting Microorganisms (NIMs) in Males and Females of the Plant Species Silene latifolia (= Silene alba)"

Professional Service

USCLancaster Committee Assignments:

  • USCL Faculty Organization Vice-Chair (2009-2011)
  • USCL Faculty Organization Secretary (2007-2009)
  • USCL Welfare and Grievance Committee (Chair, 2009-2011; member 2013-present)
  • Admissions, Petitions, and Grade Change Committee (2009-present)
  • Library Committee (2006-present), Chair 2006-2008
  • Honors Day Committee (2006-present)
  • Administrative Safety Committee (2006-2007)

USC System Committees:

  • USC Regional Campuses Faculty Senate, Welfare Committee (Full Spring 2008-Spring 2013; alternate Fall 2007-Spring 2008), Chair of Welfare Commitee 2010-2011.

Other System Services:

  • Judge at Discovery Day at USCC (oral presentations in 2011; posters in 2013)
  • USCL's Junior Scholar's Day (Oct 2008)
  • Judge in USCL's Honor's Bowl
  • USC Magellan ad hoc Scholar Reviewer (2007)

Community Service :

  • Lancaster County Science Fair Liasison (2007-present)

To the Profession:

  • 2011 Reviewer for Microbial Ecology, two articles.
  • 2011 Reviewer for FEMS Micriobiology Ecology, one article.
  • 2008 and 2011 Planting Science Program, Botanical Society of America program.
  • 2007 & 2008 Peer reviewed and evaluated labs at Association for Biological Laboratory Education Conference.

Professional Affiliations
Association for Biological Laboratory Education (2006-present)
Assocaition of Southeastern Biologists (2012-present)
Botanical Society of America (2005-2012)
Microbiological Society of America (2005-present)
Sigma Xi (2007-present)

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